Morphological and Proteomic Evaluation of Zea Mays in Response to Osmotic Stress
نویسندگان
چکیده
Introduction: Drought is the main abiotic stress responsible for crop loss worldwide. Maize ( Zea mays L.) a widely grown drought-sensitive used as staple food by growing population. Therefore, it imperative to assess molecular mechanisms behind drought response and tolerance in maize. Transcriptomic profiling of responsive pathways various crops appeared be an unreliable approach due post-transcriptional modifications, while there limited published data on osmotic-stress Hence our study aimed at osmotic proteins augmented their associated morphological features Z . Materials Methods: In this regard, proteomic investigations were carried out 16-day maize seedlings exposed 5% (w/v) 10% polyethylene glycol(PEG) induce osmotic-stress. Proteomics (one-dimensional (1D) two-dimensional (2D) gel electrophoresis) compared differential protein abundance between controls stressed plants. Results: Morphological parameters such plant growth, height, shoot diameter, leaf area, colour highly affected with PEG treatment untreated ones. Molecular evaluation 1D electrophoresis revealed that separated patterns expressed experiments than controls. Using 2D electrophoresis, total seven eight spots experimental plants under respectively control only one protein. Increased resulted greater number abundance. Conclusion: This has successfully profiled efficiency tools qualitative detection from
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ژورنال
عنوان ژورنال: The Open Biotechnology Journal
سال: 2021
ISSN: ['1874-0707']
DOI: https://doi.org/10.2174/1874070702115010019